RobotEvo
pcr
Contents:
Why RobotEvo?
Users of RobotEvo
How to run an existing protocol?
How to modify an existing protocol?
How to write a new protocol?
API
Principal API: Protocol steps
Reagent
- a fundamental concept
Worktable and labwares
RobotEvo “modes” for execution of basic instructions
Robots and arms
Examples:
RobotEvo
Docs
»
Index
Edit on GitHub
Index
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A
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C
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D
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E
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F
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G
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H
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I
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K
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L
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M
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N
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O
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P
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Q
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R
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S
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T
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U
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V
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W
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X
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Y
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Z
_
__init__() (evo_mode.Comments method)
(evo_mode.Multiple method)
(evo_mode.Script method)
(evo_mode.ToFile method)
(evo_mode.iRobot method)
(instructions.RoMa method)
(instructions.activate_PMP method)
(instructions.active_Wash method)
(instructions.aspirate method)
(instructions.comment method)
(instructions.deactivate_PMP method)
(instructions.detect_Liquid method)
(instructions.dispense method)
(instructions.dropDITI method)
(instructions.execute method)
(instructions.execute_VBscript method)
(instructions.export method)
(instructions.getDITI method)
(instructions.getDITI2 method)
(instructions.group method)
(instructions.group_end method)
(instructions.mix method)
(instructions.moveLiha method)
(instructions.notification method)
(instructions.pickUp_DITIs method)
(instructions.pickUp_DITIs2 method)
(instructions.pickUp_ZipTip method)
(instructions.set_DITI_Counter method)
(instructions.set_DITI_Counter2 method)
(instructions.set_DITIs_Back method)
(instructions.startTimer method)
(instructions.subroutine method)
(instructions.transfer_rack method)
(instructions.userPrompt method)
(instructions.variable method)
(instructions.waitTimer method)
(instructions.wash_tips method)
(instructions.waste method)
(instructions_Te_MagS.Te_MagS_ActivateHeater method)
(instructions_Te_MagS.Te_MagS_DeactivateHeater method)
(instructions_Te_MagS.Te_MagS_Execution method)
(instructions_Te_MagS.Te_MagS_Execution.Parametr method)
(instructions_Te_MagS.Te_MagS_Execution.command method)
(instructions_Te_MagS.Te_MagS_Execution.incub method)
(instructions_Te_MagS.Te_MagS_Execution.mix method)
(instructions_Te_MagS.Te_MagS_Execution.move method)
(instructions_Te_MagS.Te_MagS_Execution.wait method)
(instructions_Te_MagS.Te_MagS_MoveToPosition method)
(labware.Carrier method)
(labware.Carrier.Type method)
(labware.Carrier.Types method)
(labware.Cuvette method)
(labware.CuvetteType method)
(labware.DITIrack method)
(labware.DITIrackType method)
(labware.DITIrackTypeSeries method)
(labware.DITIwaste method)
(labware.DITIwasteType method)
(labware.Frezeer method)
(labware.Labware method)
(labware.Labware.Position method)
(labware.Labware.Type method)
(labware.Labware.Type.Series method)
(labware.LiquidClass method)
(labware.LiquidClassDefault method)
(labware.LiquidClassDerived method)
(labware.LiquidClasses method)
(labware.NoFreeWells method)
(labware.Tip method)
(labware.Well method)
(labware.Well.Action method)
(labware.WorkTable method)
(labware.WorkTable.File method)
(labware.WorkTable.Location method)
(labware.usedTip method)
(protocol_steps.Executable method)
(protocol_steps.Pipeline method)
(protocol_steps.Protocol method)
(reagent.Dilution method)
(reagent.DilutionComponentReagent method)
(reagent.MixComponent method)
(reagent.MixComponentReagent method)
(reagent.MixReagent method)
(reagent.NoReagentFound method)
(reagent.PCRMasterMix method)
(reagent.PCRMasterMixReagent method)
(reagent.PCReaction method)
(reagent.PCReactionReagent method)
(reagent.PCRexperiment method)
(reagent.PCRexperimentRtic method)
(reagent.PreMixComponent method)
(reagent.PreMixReagent method)
(reagent.Primer method)
(reagent.PrimerMix method)
(reagent.PrimerMixComponent method)
(reagent.PrimerMixReagent method)
(reagent.PrimerReagent method)
(reagent.Reaction method)
(reagent.Reagent method)
(robot.Arm method)
(robot.Robot method)
A
action() (instructions.aspirate static method)
(instructions.detect_Liquid static method)
(instructions.dispense static method)
(instructions.mix static method)
actions (instructions.waste attribute)
(labware.Well attribute)
(labware.conectedWell attribute)
activate_PMP (class in instructions)
activate_waste_1 (instructions.waste attribute)
activate_waste_2 (instructions.waste attribute)
activate_waste_3 (instructions.waste attribute)
active_Wash (class in instructions)
actualize_robot_state() (instructions.dropDITI method)
(instructions.RoMa method)
(instructions.getDITI2 method)
(instructions.mix method)
(instructions.pickUp_DITIs method)
(instructions.pickUp_DITIs2 method)
(instructions.set_DITI_Counter method)
(instructions.set_DITI_Counter2 method)
(instructions.set_DITIs_Back method)
(instructions.transfer_rack method)
add() (labware.Labware.Type.Series method)
add_labware() (labware.Carrier method)
(labware.WorkTable method)
add_mode() (evo_mode.Multiple method)
add_new_labware() (labware.WorkTable method)
add_template() (evo_mode.Script method)
add_type() (labware.Carrier.Types method)
AdvancedWorkList (class in evo_mode)
Arm (class in robot)
aspirate (class in instructions)
Aspirate (instructions_Te_MagS.Te_MagS_MoveToPosition attribute)
(robot.Arm attribute)
aspirate() (protocol_steps.Protocol method)
aspirate_one() (protocol_steps.Protocol method)
autoselect() (labware.Cuvette method)
(labware.Labware method)
C
Carrier (class in labware)
Carrier.Type (class in labware)
Carrier.Types (class in labware)
check_list() (protocol_steps.Protocol method)
check_reagent_level() (protocol_steps.Protocol method)
check_reagents_levels() (protocol_steps.Protocol method)
clearSelection() (labware.Labware method)
close_gripper (instructions.RoMa attribute)
COM_automation (class in evo_mode)
comment (class in instructions)
comment() (protocol_steps.Protocol method)
Comments (class in evo_mode)
components (reagent.Dilution attribute)
(reagent.MixReagent attribute)
(reagent.PreMixReagent attribute)
conectedWell (class in labware)
consolidate() (protocol_steps.Protocol method)
Continues (instructions.subroutine attribute)
count_tips() (in module labware)
create() (labware.Labware static method)
create_labware() (labware.CuvetteType method)
(labware.DITIrackType method)
(labware.DITIwasteType method)
(labware.Labware.Type method)
create_series() (labware.DITIrackType method)
(labware.Labware.Type method)
cur_arm() (robot.Robot method)
cur_worktable (labware.WorkTable attribute)
current (robot.Robot attribute)
Cuvette (class in labware)
CuvetteType (class in labware)
D
dbase (instructions.export attribute)
deactivate_all_wastes (instructions.waste attribute)
deactivate_PMP (class in instructions)
deactivate_system (instructions.waste attribute)
def_versions() (protocol_steps.Executable method)
Detect (robot.Arm attribute)
detect_Liquid (class in instructions)
Dilution (class in reagent)
DilutionComponentReagent (class in reagent)
dispense (class in instructions)
Dispense (instructions_Te_MagS.Te_MagS_MoveToPosition attribute)
(robot.Arm attribute)
dispense() (protocol_steps.Protocol method)
dispense_one() (protocol_steps.Protocol method)
distribute() (protocol_steps.Protocol method)
DiTi (robot.Arm attribute)
DITIrack (class in labware)
DITIrackType (class in labware)
DITIrackTypeSeries (class in labware)
DITIwaste (class in labware)
DITIwasteType (class in labware)
done() (evo_mode.Mode method)
(evo_mode.Multiple method)
(evo_mode.ToFile method)
drop_tip() (protocol_steps.Protocol method)
drop_tips() (protocol_steps.Protocol method)
drop_tips_executed() (robot.Robot method)
drop_tips_test() (robot.Robot method)
dropDITI (class in instructions)
E
eject_tips_executed() (robot.Arm method)
eject_tips_test() (robot.Arm method)
encoding (evo_mode.Mode attribute)
evo_mode (module)
excel (instructions.export attribute)
exec() (evo_mode.AdvancedWorkList method)
(evo_mode.Comments method)
(evo_mode.Mode method)
(evo_mode.Multiple method)
(evo_mode.Script method)
(evo_mode.StdOut method)
(evo_mode.ToFile method)
(evo_mode.ToString method)
(evo_mode.iRobot method)
(instructions.activate_PMP method)
(instructions.deactivate_PMP method)
(instructions.dropDITI method)
(instructions.getDITI2 method)
Executable (class in protocol_steps)
execute (class in instructions)
execute_VBscript (class in instructions)
export (class in instructions)
F
File_import (instructions.variable attribute)
fill() (labware.DITIrack method)
find_free_wells() (labware.Labware method)
find_new_tips() (labware.DITIrack method)
(labware.DITIrackTypeSeries method)
Fixed (robot.Arm attribute)
Fixed_value (instructions.variable attribute)
Frezeer (class in labware)
G
get_current_labware() (labware.WorkTable method)
get_DITI_series() (labware.WorkTable method)
get_labware() (labware.WorkTable method)
get_tips() (protocol_steps.Protocol method)
get_tips_executed() (robot.Robot method)
getDITI (class in instructions)
getDITI2 (class in instructions)
getLabware() (in module labware)
getMoreTips_test() (robot.Arm method)
getTips_test() (robot.Arm method)
(robot.Robot method)
global_z_travel (instructions.moveLiha attribute)
grid_carrier_line() (labware.WorkTable.File method)
group (class in instructions)
group_end (class in instructions)
H
home_position (instructions.RoMa attribute)
I
ids (reagent.PCRMasterMix attribute)
(reagent.Primer attribute)
(reagent.PrimerMix attribute)
ids_synt (reagent.Primer attribute)
Incubation (instructions_Te_MagS.Te_MagS_MoveToPosition attribute)
init_system (instructions.waste attribute)
init_vol() (reagent.PreMixReagent method)
(reagent.Reagent method)
init_wells() (labware.Cuvette method)
(labware.Labware method)
initialize() (protocol_steps.Executable method)
(protocol_steps.Protocol method)
Instance (instructions.variable attribute)
instructions (module)
instructions_Te_MagS (module)
iRobot (class in evo_mode)
K
key_words (reagent.Primer attribute)
(reagent.PrimerMix attribute)
L
Labware (class in labware)
labware (module)
Labware.Position (class in labware)
Labware.Type (class in labware)
Labware.Type.Series (class in labware)
labware_series (labware.WorkTable attribute)
LiquidClass (class in labware)
LiquidClassDefault (class in labware)
LiquidClassDerived (class in labware)
LiquidClasses (class in labware)
log() (labware.Well method)
lotus (instructions.export attribute)
M
make_pre_mix() (protocol_steps.Protocol method)
min_num_of_replica() (reagent.Reagent method)
min_vol() (reagent.Reagent method)
mix (class in instructions)
mix() (protocol_steps.Protocol method)
mix_reagent() (protocol_steps.Protocol method)
MixComponent (class in reagent)
MixComponentReagent (class in reagent)
mixes (reagent.PCRexperiment attribute)
(reagent.PCRexperimentRtic attribute)
MixReagent (class in reagent)
Mode (class in evo_mode)
mount_more_tips_executed() (robot.Arm method)
mount_tips_executed() (robot.Arm method)
move_labware_executed() (robot.Robot method)
move_relative (instructions.RoMa attribute)
move_to (instructions.RoMa attribute)
moveLiha (class in instructions)
moveParallel() (labware.Labware method)
Multiple (class in evo_mode)
N
names (reagent.PCRMasterMix attribute)
(reagent.Primer attribute)
(reagent.PrimerMix attribute)
need_vol (reagent.Reagent attribute)
newline (evo_mode.Mode attribute)
newOffset() (labware.Labware method)
newPosition() (labware.Labware method)
NoFreeWells
NoReagentFound
notification (class in instructions)
Numeric (instructions.variable attribute)
O
offset() (labware.Labware method)
offsetAtParallelMove() (labware.Labware method)
offsetFromName() (labware.Labware method)
open() (evo_mode.ToFile method)
open_gripper (instructions.RoMa attribute)
P
paradox (instructions.export attribute)
parallelOrder() (labware.Labware method)
parse_file() (labware.Carrier.Types method)
parse_worktable_file() (labware.WorkTable method)
pcr_exp (reagent.PCRexperimentRtic attribute)
pcr_reactions (reagent.PCRexperiment attribute)
PCReaction (class in reagent)
PCReactionReagent (class in reagent)
PCRexperiment (class in reagent)
PCRexperimentRtic (class in reagent)
PCRMasterMix (class in reagent)
PCRMasterMixReagent (class in reagent)
pick_up() (labware.DITIrack method)
pick_up_tip() (protocol_steps.Protocol method)
pick_up_tips_executed() (robot.Robot method)
pickUp_DITIs (class in instructions)
pickUp_DITIs2 (class in instructions)
pickUp_ZipTip (class in instructions)
Pipeline (class in protocol_steps)
pipette_executed() (robot.Arm method)
(robot.Robot method)
pos_global_z_travel (instructions.moveLiha attribute)
pos_local_z_travel (instructions.moveLiha attribute)
posAtParallelMove() (labware.Labware method)
position() (labware.Labware method)
PreMixComponent (class in reagent)
PreMixReagent (class in reagent)
preserve_tips() (robot.Robot method)
Primer (class in reagent)
PrimerMix (class in reagent)
PrimerMixComponent (class in reagent)
PrimerMixReagent (class in reagent)
PrimerReagent (class in reagent)
Protocol (class in protocol_steps)
protocol_steps (module)
ProtocolLogicPippetingError
put() (labware.Labware method)
put_min_vol() (reagent.Reagent method)
Q
quattro (instructions.export attribute)
R
Re_suspension (instructions_Te_MagS.Te_MagS_MoveToPosition attribute)
Reaction (class in reagent)
Reagent (class in reagent)
reagent (labware.conectedWell attribute)
(labware.Well attribute)
(module)
reagents (labware.WorkTable attribute)
refill_next_rack() (labware.DITIrackTypeSeries method)
remove() (labware.Labware.Type.Series method)
replace_with_new() (labware.WorkTable method)
retire_labware() (labware.WorkTable method)
retire_new_tips() (labware.DITIrack method)
(labware.DITIrackTypeSeries method)
reuse_tips() (protocol_steps.Protocol method)
(robot.Robot method)
Robot (class in robot)
robot (module)
RoMa (class in instructions)
RoMa_1 (instructions.RoMa attribute)
RoMa_2 (instructions.RoMa attribute)
Run (instructions.variable attribute)
run() (protocol_steps.Executable method)
(protocol_steps.Protocol method)
S
samples (reagent.PCRexperiment attribute)
Script (class in evo_mode)
(instructions.variable attribute)
ScriptBody (class in evo_mode)
select() (labware.Labware method)
(labware.Well method)
selectAll() (labware.Labware method)
selected() (labware.Labware method)
selected_wells() (labware.Labware method)
selectOnly() (labware.Labware method)
seqs (reagent.Primer attribute)
set_allow_air() (robot.Robot method)
set_as_current() (evo_mode.iRobot method)
(robot.Robot method)
set_back() (labware.DITIrack method)
set_current() (labware.WorkTable method)
set_current_next_to() (labware.Labware.Type.Series static method)
set_def_DiTi() (labware.WorkTable method)
set_defaults() (protocol_steps.Executable method)
(protocol_steps.Protocol method)
set_DITI_Counter (class in instructions)
set_DITI_counter() (labware.DITIrack method)
set_DITI_Counter2 (class in instructions)
set_DITIs_Back (class in instructions)
set_drop_tips() (protocol_steps.Protocol method)
(robot.Robot method)
set_file() (evo_mode.ToFile method)
set_first_pos() (labware.WorkTable method)
set_first_tip() (protocol_steps.Protocol method)
set_next() (labware.Labware.Type.Series method)
set_template() (evo_mode.Script method)
set_tips_back_executed() (robot.Robot method)
set_worktable() (robot.Robot method)
show_check_list() (protocol_steps.Protocol method)
show_next() (labware.Labware.Type.Series method)
show_next_to() (labware.Labware.Type.Series static method)
size() (labware.Labware.Type method)
startTimer (class in instructions)
StdOut (class in evo_mode)
String (instructions.variable attribute)
subroutine (class in instructions)
T
targets (reagent.PCRexperiment attribute)
Te_Mag (class in labware)
Te_MagS_ActivateHeater (class in instructions_Te_MagS)
Te_MagS_DeactivateHeater (class in instructions_Te_MagS)
Te_MagS_Execution (class in instructions_Te_MagS)
Te_MagS_Execution.command (class in instructions_Te_MagS)
Te_MagS_Execution.incub (class in instructions_Te_MagS)
Te_MagS_Execution.mix (class in instructions_Te_MagS)
Te_MagS_Execution.move (class in instructions_Te_MagS)
Te_MagS_Execution.Parametr (class in instructions_Te_MagS)
Te_MagS_Execution.wait (class in instructions_Te_MagS)
Te_MagS_MoveToPosition (class in instructions_Te_MagS)
text_with_delimiters (instructions.export attribute)
Tip (class in labware)
tips() (protocol_steps.Protocol method)
to_name() (labware.Labware.Position method)
ToFile (class in evo_mode)
ToString (class in evo_mode)
transfer() (protocol_steps.Protocol method)
transfer_rack (class in instructions)
types (labware.Labware attribute)
U
use_max_speed (instructions.RoMa attribute)
use_preserved_tips() (robot.Robot method)
use_tips_executed() (robot.Robot method)
use_version() (protocol_steps.Executable method)
use_xyzSpeed (instructions.RoMa attribute)
usedTip (class in labware)
user_prompt() (protocol_steps.Protocol method)
User_query (instructions.variable attribute)
userPrompt (class in instructions)
V
validate_arg() (instructions.activate_PMP method)
(instructions.RoMa method)
(instructions.active_Wash method)
(instructions.aspirate method)
(instructions.comment method)
(instructions.deactivate_PMP method)
(instructions.dispense method)
(instructions.dropDITI method)
(instructions.execute method)
(instructions.execute_VBscript method)
(instructions.export method)
(instructions.getDITI method)
(instructions.getDITI2 method)
(instructions.group method)
(instructions.group_end method)
(instructions.mix method)
(instructions.moveLiha method)
(instructions.notification method)
(instructions.pickUp_DITIs method)
(instructions.pickUp_DITIs2 method)
(instructions.set_DITI_Counter method)
(instructions.set_DITI_Counter2 method)
(instructions.set_DITIs_Back method)
(instructions.startTimer method)
(instructions.subroutine method)
(instructions.transfer_rack method)
(instructions.userPrompt method)
(instructions.variable method)
(instructions.waitTimer method)
(instructions.wash_tips method)
(instructions.waste method)
(instructions_Te_MagS.Te_MagS_ActivateHeater method)
(instructions_Te_MagS.Te_MagS_DeactivateHeater method)
(instructions_Te_MagS.Te_MagS_Execution method)
(instructions_Te_MagS.Te_MagS_MoveToPosition method)
variable (class in instructions)
vector (class in instructions)
vol (labware.conectedWell attribute)
(labware.Well attribute)
W
Waits (instructions.subroutine attribute)
Waits_previous (instructions.subroutine attribute)
waitTimer (class in instructions)
wash_tips (class in instructions)
waste (class in instructions)
waste() (labware.DITIwaste method)
Well (class in labware)
Well.Action (class in labware)
wellSelectionStr() (labware.Labware method)
where_are_preserved_tips() (robot.Robot method)
where_preserve_tips() (robot.Robot method)
WorkTable (class in labware)
WorkTable.File (class in labware)
WorkTable.Location (class in labware)
write() (labware.WorkTable.File method)
X
x_move (instructions.moveLiha attribute)
Y
y_move (instructions.moveLiha attribute)
Z
z_dispense (instructions.moveLiha attribute)
z_max (instructions.moveLiha attribute)
z_move (instructions.moveLiha attribute)
z_start (instructions.moveLiha attribute)
z_travel (instructions.moveLiha attribute)
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